Expertise

Medical Microbiology

Research Interests

Global health, infectious disease, enteric infections, gut microbiome, mucosal immunity and vaccine response.

Postdoctoral Training

7/2018-7/2019 Clinical Fellow, Medical Microbiology, Brigham and Women’s Hospital
7/2016-7/2018 Research Fellow, Department of Pathology & Laboratory Medicine, LCOM, University of Vermont
7/2012 – 7/2016 Resident Physician, Anatomic and Clinical Pathology, Department of Pathology & Laboratory Medicine, LCOM, University of Vermont

Education

2012 M.D. Larner College of Medicine, University of Vermont
2005 B.A. Cum Laude, University of Vermont

Academic Appointments

2019-present Assistant Professor, Department of Pathology & Laboratory Medicine, Larner College of Medicine, University of Vermont
2012-2018 Clinical Instructor, Larner College of Medicine, University of Vermont

Awards and Honors

2018 Center of Expertise in Global & Community Health, Travel Grant, Harvard Medical School Project: Spreading Antimicrobial Resistance Education
2015 Inauguration into The Teaching Academy, Larner College of Medicine, University of Vermont

Publications

“Variant mutation G215C in SARS-CoV-2 nucleocapsid enhances viral infection via altered genomic encapsidation.” Kubinski HC, Despres HW, Johnson BA, Schmidt MM, Jaffrani SA, Turner AH, Fanuele CD, Mills MG, Lokugamage KG, Dumas CM, Shirley DJ, Estes LK, Pekosz A, Crothers JW, Roychoudhury P, Greninger AL, Jerome KR, Di Genova BM, Walker DH, Ballif BA, Ladinsky MS, Bjorkman PJ, Menachery VD, Bruce EA. PLoS Biology. 2025 Apr 29;23(4):e3003115. doi: 10.1371/journal.pbio.3003115. eCollection 2025 Apr. PMID: 40299982

"Recent advances in enterotoxin vaccine adjuvants." Crothers JW, Norton EB. Current Opinion in Immunology. 2023 Dec;85:102398. doi: 10.1016/j.coi.2023.102398. Epub 2023 Nov 16. PMID: 37976963

"Determining the impact of vaccination on SARS-CoV-2 RT-PCR cycle threshold values and infectious viral titres." Peterson KL, Snyder JP, Despres HW, Schmidt MM, Eckstrom KM, Unger AL, Carmolli MP, Sevigny JL, Shirley DJ, Dragon JA, Thomas WK, Bruce EA, Crothers JW. Access Microbiology. 2023 Oct 20;5(10):000597.v3. doi: 10.1099/acmi.0.000597.v3. PMID: 37970082; PMCID: PMC10634488

"Viral infectivity in paediatric SARS-CoV-2 clinical samples does not vary by age." Schmidt MM, Despres HW, Shirley DJ, Bose ME, McCaul KC, Crothers JW, Henrickson KJ, Lee B, & Bruce EA. Access Microbiology. 2023 5(5), acmi000547.v4. https://doi.org/10.1099/acmi.0.000547.v4 h-index: 9

"Prospective Genomic Surveillance Reveals Cryptic MRSA Outbreaks with Local to International Origins among NICU Patients." Worley JN, Crothers JW, Wolfgang WJ, Venkata SLG, Hoffmann M, Jayeola V, Klompas M, Allard M, Bry L. Journal of Clinical Microbiology. 2023 Apr 6:e0001423. doi: 10.1128/jcm.0014-23. Epub ahead of print. PMID: 37022157

Corrigendum to "Intradermal fractional-dose inactivated polio vaccine (fIPV) adjuvanted with double mutant Enterotoxigenic Escherichia coli heat labile toxin (dmLT) is well-tolerated and augments a systemic immune response to all three poliovirus serotypes in a randomized active-controlled trial" [Vaccine 40(19) (2022) 2705-2713]. Crothers JW, Ross Colgate E, Cowan KJ, Dickson DM, Walsh M, Carmolli M, Wright PF, Norton EB, Kirkpatrick BD. Vaccine. 2022 May 31;40(25):3481. doi: 10.1016/j.vaccine.2022.05.001. Epub 2022 May 6. PMID: 35534312

“Effectiveness and safety of fecal microbiota transplantation for Clostridioides difficile infection: Results from a 5,344 patient cohort study.” Osman M, Budree S, Kelly CR, Panchal P, Allegretti JR, Kassam Z; OpenBiome collaborators, Olesen SW, Ramakrishna B, Dubois N, O'Brien K, Fischer M, Stollman N, Hays RA, Kelly CP, Amaratunga K, Qazi T, Crothers JW, Abend A, Bougas M, Burns L, Decaille-Hodge I, Dickens M, Edelstein C, Gabdrakhmanova D, Landry R, Ling K, Martin D, Medina G, Mendolia G, Muñoz R, Rao S, Seng M, Smith M, Stehler L, Yoder K, Zellmer C. Gastroenterology. 2022 Apr 7:S0016-5085(22)00351-1. doi: 10.1053/j.gastro.2022.03.051. Online ahead of print. PMID: 35398345 No abstract available.

“Intradermal fractional-dose inactivated polio vaccine (fIPV) adjuvanted with double mutant Enterotoxigenic Escherichia coli heat labile toxin (dmLT) is well-tolerated and augments a systemic immune response to all three poliovirus serotypes in a randomized placebo-controlled trial.” Crothers JW, Ross Colgate E, Cowan KJ, Dickson DM, Walsh M, Carmolli M, Wright PF, Norton EB, Kirkpatrick BD. Vaccine. 2022 Mar 30:S0264-410X(22)00367-X. doi: 10.1016/j.vaccine.2022.03.056. Online ahead of print. PMID: 35367069

“An international, interlaboratory ring trial confirms the feasibility of an extraction-less "direct" RT-qPCR method for reliable detection of SARS-CoV-2 RNA in clinical samples.” Mills MG, Bruce E, Huang ML, Crothers JW, Hyrien O, Oura CAL, Blake L, Brown Jordan A, Hester S, Wehmas L, Mari B, Barby P, Lacoux C, Fassy J, Vial P, Vial C, Martinez JRW, Oladipo OO, Inuwa B, Shittu I, Meseko CA, Chammas R, Santos CF, Dionísio TJ, Garbieri TF, Parisi VA, Mendes-Correa MC, de Paula AV, Romano CM, Góes LGB, Minoprio P, Campos AC, Cunha MP, Vilela APP, Nyirenda T, Mkakosya RS, Muula AS, Dumm RE, Harris RM, Mitchell CA, Pettit S, Botten J, Jerome KR. PLoS One. 2022 Jan 13;17(1):e0261853. doi: 10.1371/journal.pone.0261853. eCollection 2022.PMID: 35025926 Free PMC article.

“Dynamic Colonization of Microbes and Their Functions after Fecal Microbiota Transplantation for Inflammatory Bowel Disease.” Chu ND, Crothers JW, Nguyen LTT, Kearney SM, Smith MB, Kassam Z, Collins C, Xavier R, Moses PL, Alm EJ. mBio. 2021 Jul 20:e0097521. doi: 10.1128/mBio.00975-21. Online ahead of print.PMID: 34281401